Mudukaki started practising nursing in 1952 and worked in Iganga and Tororo district hospitals before she was deployed at Mulago National Referral Hospital. Upon her retirement, Mudukaki worked as a school nurse at Buddo Senior Secondary School for six years, before opening a private clinic that she later closed after developing cardiac challenges.
90 year old, Alice Nakanyike Mudukaki, holds a certificate of appreciation awarded to her by nurses, under their umbrella body of Uganda Nurses and Midwives Union-UNMU.
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